3-Methylhistidine Analysis

3-methylhistinde (3MH) expertise

MTI provides 3-methylhistidine (3MH) analysis. Laboratory director Dr. John A. Rathmacher has had extensive experience working with amino acids and specializes in the analysis of 3MH. Dr. Rathmacher has over 10 refereed publications and 20 abstracts regarding 3MH and its usefulness in accessing muscle protein turnover. MTI can analyze various samples, including lab animals, humans, cell cultures and tissue hydrolysis.

Urinary 3-methylhistidine

Urinary 3MH is important in the measurement of skeletal muscle (myofibrillar) protein breakdown. The procedure of measuring urinary 3MH entails a quantitative collection of urine, with the assumption that no metabolism of 3MH has occurred after being released from actin and myosin. If the precursor pool of 3MH (3MH bound to muscle protein) can be determined, then a fractional breakdown rate can be calculated, otherwise the measured amount of urinary 3MH can be used as an index of protein degradation.

Procedure

3MH will be quantified using an internal standard (ISTD) method. A dueterated-3MH molecule will be used for the procedure of the analysis and quantification as the ISTD. 3MH will be analyzed by gas chromatography/mass spectrometry in the laboratory using procedures developed at MTI.1, 2 All methods developed in the laboratory adhere to Good Laboratory Practices (GLP). These practices also utilize precision, accuracy and quality control requirements that have been outlined in our Standard Operating Procedures (SOP). All the data obtained during the procedure will be recorded in an Excel spreadsheet as a value of nmol/mL, unless the 24-h urine value is provided prior to the analysis. If the customer provides this value, the results will instead be recorded as µmol/mL.